University of Wisconsin - Madison

Position Vacancy Listing

PVL # 235740

Position Summary:
This is an opportunity to join an established lab that collaborates extensively with other virology labs on campus and beyond to study the role of viruses, particularly Epstein-Barr virus in cancer. The primary responsibility will be to learn to construct and validate mutant viruses that are suitable for use in cell culture, in vitro, and animal models. While the lab has established protocols for these procedures, the successful individual will be interested in innovation and be an excellent problem solver.

The School of Medicine and Public Health has a deep and profound commitment to diversity both as an end in itself but also as a
valuable means for eliminating health disparities. As such, we strongly encourage applications from candidates who foster and
promote the values of diversity and inclusion.

Principal duties:
25% Conducts research experiments according to established research protocols with moderate impact to the project(s). Collects data and monitors test results
- Support and conduct independent laboratory research under the guidance of the PI, involving molecular biology (cloning, mutagenesis, PCR), mammalian cell culture (maintaining lines, transfection, transduction, infection), and protein biochemistry (immunoprecipitation, western blotting, and chromatin immunoprecipitation (ChIP)).

10% Provides operational guidance on day-to-day activities of unit or program staff and/or student workers
- Collaborate with students and other lab members and participate in training them as appropriate.

30% Participates in the development, interpretation, and implementation of research methodology and materials
- Design mutagenesis approaches in collaboration with the PI to create scarless deletions, insertions, and point mutations in EBV BACmids.
- Troubleshoot and redesign experiments with input as required from the PI for overall project goals.
- Verify and validate mutants using conventional techniques including Sanger sequencing, PCR, and restriction digests.

10% Reviews, analyzes, and interprets data and/or documents results for presentations and/or reporting to internal and external audiences
- Participate in weekly lab meetings and other scientific venues, giving summaries of current work and future directions.

15% Performs literature reviews and writes reports
- Maintain a laboratory notebook documenting experimental details, so that experiments can be reproduced later. Share results in frequent informal discussions with PI, regular group meetings, and occasional formal presentations in the broader scientific community.

10% Operates, cleans, and maintains organization of research equipment and research area. Tracks inventory levels and places replenishment orders
- Order supplies, maintain an inventory of laboratory reagents, conduct routine maintenance of laboratory equipment, and update established lab protocols.

© 2019 Board of Regents of the University of Wisconsin System